Our research
Alphalyse is the world's leading expert in host cell protein analysis by mass spectrometry. We have been at the forefront of protein analysis research for over two decades - science comes first in everything we do.
Our goal is to support the development of superior biologics through better analytics. Our research on drug product characterization and protein impurity analysis by mass spectrometry provides biopharmaceutical developers with a comprehensive understanding of their therapeutics, improving drug quality and patient safety.
Research and collaboration partners
Collaborations with clients
Alphalyse is a global leader in protein analysis by mass spectrometry. Our research focuses on solving pressing analytical challenges in the biopharmaceutical industry.
By prioritizing client collaborations, we ensure that our research meets real-world needs. Our work spans therapeutic protein characterization, impurity identification and quantification, and HCP coverage analysis, to support drug development processes and improve therapeutic outcomes.
Our client collaborations have resulted in the world's first GMP-compliant MS-based HCP analysis for release testing.
EU and DK funded innovation
Since 2014, Alphalyse has received €1.4M in funding from Innovation Fund Denmark, the Market Development Fund, and Eurostars for development of novel HCP analysis by mass spectrometry, methods for validation, and high-throughput antibody characterization.
These projects have been conducted in collaboration with the Protein Research Group at the University of Southern Denmark, and biotherapeutics developers in Europe and the USA.
Non-profit organization partnerships
Alphalyse is proud to collaborate with several non-profit organizations, including the US Pharmacopeia, the Biopharmaceutical Emerging Best Practices Association (BEBPA), and Life Science Fyn.
These collaborations provide platforms for Alphalyse to share research results and contribute insights in support of broader efforts to improve drug product quality, efficacy, safety, and overall industry standards for the benefit of patients and the biopharmaceutical community.
Academic research
Alphalyse has a long-standing tradition of working with leading university institutions to bridge the gap between cutting-edge academic research and real-world industry applications.
Together with the University of Southern Denmark, Alphalyse offers student internships, BSc and MSc thesis projects, and PhD programs that give researchers and students hands-on experience with advanced protein analysis techniques, drive scientific breakthroughs, and educate the next generation of proteomics experts.
20 years of innovation
Our protein analysis research
Since 2002, Alphalyse has been a pioneer in advanced protein analytics, continuously refining techniques and developing innovative methods to meet the evolving needs of the biopharmaceutical industry.
Our research has contributed to shaping mass spectrometry-based analysis of biologics and residual impurities. Find our most referenced publications below.
Paper: HCP Coverage by Immunocapture and LC-MS/MS
Poster: Sensitive MS-assay for detecting polysorbate-degrading HCPs in mAbs
Paper: Monitoring process-related impurities in biologics - HCP analysis
Poster: ID & quantify individual HCPs using SWATH® LC-MS
Protocol: Silver staining of gels optimized for LC-MS protein identification
The Alphalyse HCP database
We have access to our own unique database containing information on all HCPs we have identified in client samples of recombinant
proteins, mAbs, vaccines, and gene therapy products, from every stage of development and manufacturing.
Total HCPs
Unique HCPs
Total ions
Total peptides
Unique samples
Organisms
Peer-reviewed papers with data from Alphalyse
K Pilely, SB Nielsen, A Draborg ML. Henriksen, SWK Hansen, L Skriver, E Mørtz, RR Lund
A novel approach to evaluate ELISA antibody coverage of host cell proteins—combining ELISA‐based immunocapture and mass spectrometry
Biotechnology Process, February 2020
MJ la Cour, J Mollerup, MW Berchtold MW.
ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations
Biochemical and Biophysical Research Communications, February 2007.
J Acevedo, Y Bi, J Gee, SL Khatwani.
Assessment of Adeno-Associated Virus Purity by Capillary Electrophoresis-Based Western
Molecular Therapy: Methods & Clinical Development, September 2024
V Eckardt, MC Miller, X Blanchet, R Duan, J Leberzammer, et. al.
Chemokines and galectins form heterodimers to modulate inflammation
EMBO Reports, February 2020
S Heissel, J Bunkenborg, MP Kristiansen, A Fich Holmbjerg, M Grimstrup, E Mørtz, T Kofoed, P Højrup
Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein
Protein Expression and Purification Volume 147, July 2018.
M Bielaszewska, C Rüter, A Bauwens, L Greune, K-A Jarosch, D Steil, W Zhang, X He, R Lloubes, A Fruth, K Sik Kim, MA Schmidt, U Dobrindt, A Mellmann, H Karch
Host cell interactions of outer membrane vesicle-associated virulence factors of enterohemorrhagic Escherichia coli O157: Intracellular delivery, trafficking and mechanisms of cell injury
PLOS Pathogens, February 2017
K Pilely, MR. Johansen, RR Lund, T Kofoed, TK Jørgensen, L Skriver, E Mørtz
Monitoring process-related impurities in biologics – host cell protein analysis
Analytical and Bioanalytical Chemistry, September 2021
CB Petersen, A-B Nygård, B Viuff, M Fredholm, B Aasted, J Salomonsen
Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1: Cloning, transcription, expression, mapping and identification of an NPP1/CD203a epitope for swine workshop cluster p monoclonal antibodies
Developmental & Comparative Immunology, 2007.
Casper Normann Nurup, Tamás László Czárán, Fergal P. Rattray
A chromatographic approach to understanding the plasmin-plasminogen system in acid whey
International Dairy Journal, March 2020
Linda D. Hicks, Rahul Raghavan, James M. Battisti, Michael F. Minnick
A DNA-binding peroxiredoxin of Coxiella burnetii is involved in countering oxidative stress during exponential-phase growth
Bacteriol, April 2010, 192(8), pp. 2077-84
Rohit Ruhala, Henrik Anttia, Olena Rzhepishevskaa, Nicolas Boulangerb, David R. Barberob, Sun Nyunt Waic, Bernt Eric Uhlinc, Madeleine Ramstedt
A multivariate approach to correlate bacterial surface properties to biofilm formation by lipopolysaccharide mutants of Pseudomonas aeruginosa
Colloids and Surfaces B: Biointerfaces, Volume 127, Pages 182–191, March 2015
Song T, Mika F, Lindmark B, Liu Z, Schild S, Bishop A, Zhu J, Camilli A, Johansson J, Vogel J, Wai SN
A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles
Mol Microbiol., October 2008; 70(1)
Karina Juhl Rasmussena, Mikkel-Ole Skjoedtb, Lars Vitveda, Karsten Skjoedta, Yaseelan Palarasahc
A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product
Journal of Immunological Methods Volume 444, May 2017, Pages 51–55
Susanne Erdmann, Bo Chen, Xiaoxing Huang, et al.
A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species
Extremophiles, October 2013, Vol. 18, Issue 1, pp. 51-60
Bullock, T. L., Rodríguez-Hernández, A., Corigliano, E. M., & Perona, J. J.
A rationally engineered misacylating aminoacyl-tRNA synthetase
PNAS, May 27, 2008, vol. 105, no. 21, 7428-7433
Ji-Young Kim, Taraswi Banerjee, Aurimas Vinckevicius, Qianyi Luo, J. Brandon Parker, Mairead R. Baker, Ishwar Radhakrishnan, Jian-Jun Wei, Grant D. Barish, Debabrata Chakravarti
A Role for WDR5 in Integrating 11 Phosphorylation to Lysine 4 Methylation on Histone H3 during Androgen Signaling and in Prostate Cancer
Molecular Cell, May 2014
Katrine Pilely Hansen, Mikkel-Ole Skjoedt, Christian Nielsen, Thomas Emil Andersen, Anne Åbom, Lars Vitved, Claus Koch, Karsten Skjødt, Yaseelan Palarasah
A specific assay for quantification of human C4c by use of an anti-C4c monoclonal antibody
Journal of Immunological Methods, January 2014
Luisa Bermúdez, Talía del Pozo, Bruno Silvestre Lira, Fabiana de Godoy, Irene Boos, Cecilia Romanó et al.
A Tomato Tocopherol Binding Protein Sheds Light on Intracellular α-tocopherol Metabolism in Plants
Plant and Cell Physiology, pcy191, September 2018
James M. Battisti, Linda D. Hicks, Michael F. Minnick
A unique Coxiella burnetii lipoprotein involved in metal binding (LimB)
Microbiology, 2011, vol. 157, pp. 966-976
Susanne Erdmann, Urte Scheele, Roger A. Garrett
AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition
Virology, December 2011, Vol. 421, Issue 1, pp. 61-66
Eisuke Murakami, Haiying Bao, Ralph T. Mosley, Jinfa Du, Michael J. Sofia, Phillip A. Furman
Adenosine Deaminase-like Protein 1 (ADAL1): Characterization and Substrate Specificity in the Hydrolysis of N6- or O6-Substituted Purine or 2-Aminopurine Nucleoside Monophosphates
Med. Chem., 2011, 54 (16), pp. 5902–5914
Linda Tancred, Maxim V. Telepnev, Igor Golovlev, Blanka Andersson, Henrik Andersson, Helena Lindgren, Anders Sjostedt
Administration of a donor of nitric oxide inhibits mglA expression of intracellular Francisella tularensis and counteracts phagosomal escape and subversion of TNF-alpha secretion
J Med Microbiol, June 2011
Wørzner, J. Hvannastein, S.T. Schmidt, C. Foged, I. Rosenkrands, G. K. Pedersen, D. Christensen
Adsorption of protein antigen to the cationic liposome adjuvant CAF®01 is required for induction of Th1 and Th17 responses but not for antibody induction
European Journal of Pharmaceutics and Biopharmaceutics, Volume 165, 2021, Pages 293-305, May 2021
Tobias Kromann-Hansen, Ida K. Lund, Zhuo Liu, et al.
Allosteric Inactivation of a Trypsin-Like Serine Protease by An Antibody Binding to the 37- and 70-Loops
ACS Publications Biochemistry, 2013
Stine N. Clemmensen, Lars C. Jacobsen, Sara Rørvig, Bjarke Askaa, Karin Christenson, Martin Iversen, Marianne H. Jørgensen, Maria T. Larsen, Bo van Deurs, Ole Østergaard, Niels H. Heegaard, Jack B. Cowland, Niels Borregaard
Alpha-1-antitrypsin is produced by human neutrophil granulocytes and their precursors and liberated during granule exocytosis
European Journal of Haematology, June 2011, vol. 86, Issue 6, pp. 517–530
Karina Krotova, George W. Marek, Rejean L. Wang, George Aslanidi, Brad E. Hoffman, Nazli Khodayari, Farshid N. Rouhani & Mark L. Brantly
Alpha1-antitrypsin–deficient Macrophages Have Increased Matriptase-mediated Proteolytic Activity
American Journal of Respiratory Cell and Molecular Biology, March 2017
Gert H. Hansen, Esben D. K. Pedersen, Lissi Immerdal, Lise-Lotte Niels-Christiansen, and E. Michael Danielsen
Anti-glycosyl antibodies in lipid rafts of the enterocyte brush border: a possible host defense against pathogens
Am J Physiol Gastrointest Liver Physiol, 2005, 289(6), 1100-1107
Jan Platenik, Vladimir J. Balcar, Yukio Yoneda, Barbara Mioduszewska, Richard Buchal, Radovan Hynek, Lukasz Kilianek, Nobuyuki Kuramoto, Grzegorz Ilczynski, Kiyokazu Ogita, Yoichi Nakamura, Leszek Kaczmarek
Apparent presence of Ser133-phosphorylated cyclic AMP response element binding protein (pCREB) in brain mitochondria is due to cross-reactivity of pCREB antibodies with pyruvate dehydrogenase
Journal of Neurochemistry, 2005, 95, 1446–1460
Juliane Kühn, Ariane Briegel, Erhard Mörschel, Jörg Kahnt, Katja Leser, Stephanie Wick, Grant J Jensen, Martin Thanbichler
Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus
The EMBO Journal, 2010, 29, pp. 327-339
Pseudomonas Evades Immune Recognition of Flagellin in Both Mammals and Plants
Bart W. Bardoel, Sjoerd van der Ent, Michiel J. C. Pel, Jan Tommassen, Corné M. J. Pieterse, Kok P. M. van Kessel, Jos A. G. van Strijp
PLoS Pathog 7(8), August 2011
Saeid Karkehabadi, Kate E. Helmich, Thijs Kaper, Henrik Hansson, Nils Egil Mikkelsen, Mikael Gudmundsson, Kathleen Piens, Meredith Fujdala, Goutami Banerjee, John S. Scott-Craig, Jonathan D. Walton, George N. Phillips Jr., Mats Sandgren
Biochemical Characterization and Crystal Structures of a Fungal Family 3 β-Glucosidase, Cel3A from Hypocrea jecorina
The Journal of Biological Chemistry, August 2014
Johanne Mørch Jensen, Fie C. Simonsen, Amir Mastali, et al.
Biophysical characterization of the proton-coupled oligopeptide transporter YjdL
Peptides, 2012
J Albrethsen, R Bøgebo, CH Møller, J Olsen
Candidate biomarker verification: critical examination of a serum protein pattern for human colorectal cancer
PROTEOMICS – Clinical Applications Volume 6, Issue 3-4, pages 182–189, April 2012
Björn Alriksson, Shaunita H. Rose, Willem H. van Zyl, Anders Sjöde, Nils-Olof Nilvebrant, Leif J. Jönsson
Cellulase Production from Spent Lignocellulose Hydrolysates by Recombinant Aspergillus niger
Applied and Environmental Microbiology, April 2009, p. 2366-2374, Vol. 75, No. 8
Roussel-Jazédé, V., Van Gelder, P., Sijbrandi, R., Rutten, L., Otto, B. R., Luirink, J., Gros, P., Tommassen, J., & Van Ulsen, P.
Channel properties of the translocator domain of the autotransporter Hbp of Escherichia coli
Molecular Membrane Biology, April 2011, Vol. 28, No. 3, Pages 158-170 (doi:10.3109/09687688.2010.550328)
Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen, Thomas Kofoed
Characterization of a novel +70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry
Amino Acids, August 2021
Marina G. Kalyuzhnaya, Krassimira R. Hristova, Mary E. Lidstrom, and Ludmila Chistoserdova
Characterization of a Novel Methanol Dehydrogenase in Representatives of Burkholderiales: Implications for Environmental Detection of Methylotrophy and Evidence for Convergent Evolution
Journal of Bacteriology, June 2008, pp. 3817-3823
Oriana Flores, Jennifer Alcaíno, María Fernandez-Lobato, Víctor Cifuentes, Marcelo Baeza
Characterization of virus-like particles and identification of capsid proteins in Xanthophyllomyces dendrorhous
Virus Genes, Volume 50, Issue 2, pp 253-259, April 2015
Ana R. Cabrera, Kevin V. Donohue, Sayed M.S. Khalila, Daniel E. Sonenshine, R. Michael Roe
Characterization of vitellin protein in the twospotted spider mite, Tetranychus urticae
Journal of Insect Physiology, Volume 55, Issue 7, July 2009, Pages 655-661
Sækmose, S. G., Holst, R., Lottenburger, T., Ytting, H., Nielsen, H. J., Junker, P., Schlosser, A., & Sorensen, G. L.
Circadian, Week-to-Week, and Physical Exercise-Induced Variation of Serum Microfibrillar-Associated Protein 4
Biomarker Insights, vol. 16, May 2021
Girdhari M. Sharma, Andre Irsigler, Pushparani Dhanarajan, Rosalia Ayuso, Luda Bardina, Hugh A. Sampson, Kenneth H. Roux, Shridhar K. Sathe
Cloning and Characterization of 2S Albumin, Car i 1, a Major Allergen in Pecan
Agric. Food Chem., 2011, 59
Girdhari M. Sharma, Andre Irsigler, Pushparani Dhanarajan, Rosalia Ayuso, Luda Bardina, Hugh A. Sampson, Kenneth H. Roux, Shridhar K. Sathe
Cloning and Characterization of an 11S Legumin, Car i 4, a Major Allergen in Pecan
Agric. Food Chem., 2011, 59 (17), pp. 9542–9552
Darrin Kuystermans, Mohamed Al-Rubeai
cMyc increases cell number through uncoupling of cell division from cell size in CHO cells
BMC Biotechnology, 2009, 9:76
David S. Gibsona, Sarah Blelocka, Jim Currya, Sorcha Finnegana, Adrienne Healya, Caitriona Scaifeb, Catherine McAllistera, Stephen Penningtonb, Michael Dunnb, Madeleine Rooneya
Comparative analysis of synovial fluid and plasma proteomes in juvenile arthritis – Proteomic patterns of joint inflammation in early-stage disease
Journal of Proteomics, May 2009, Vol. 72, Issue 4, pp. 656-676
Alireza Shahriary, Hossein Mehrani, Mostafa Ghaneim, Shahram Parvin
Comparative proteome analysis of peripheral neutrophils from sulfur mustard-exposed and COPD patients
Journal of Immunotoxicology, May 2014
Nouhi, S., Kwaambwa, H. M., Gutfreund, P., et al.
Comparative study of flocculation and adsorption behaviour of water treatment proteins from Moringa peregrina and Moringa oleifera seeds
Sci Rep, 9, 17945, November 2019
Patrick Oeckl, Petra Steinacker, Markus Otto
Comparison of internal standard approaches for SRM analysis of alpha-synuclein in cerebrospinal fluid
Journal of Proteome Research, November 2017
Hoffmann, A., Neumann, P., Schierhorn, A., & Stubbs, M. T.
Crystallization of Spätzle, a cystine-knot protein involved in embryonic development and innate immunity in Drosophila melanogaster
Acta Cryst., 2008, F64, 707-710
Anil Koul, Luc Vranckx, Neeraj Dhar, Hinrich W.H. Göhlmann, Emre Özdemir, Jean-Marc Neefs, Melanie Schulz, Ping Lu, Ejvind Mørtz, John D. McKinney, Koen Andries & Dirk Bald
Delayed bactericidal response of Mycobacterium tuberculosis to bedaquiline involves remodelling of bacterial metabolism
Nature Communications, February 2014
Ian Gaël Rodrigue-Gervais, Hawley Rigsby, Loubna Jouan, Dominike Sauvé, Rafick-Pierre Sékaly, Bernard Willems, Daniel Lamarre
Dendritic Cell Inhibition Is Connected to Exhaustion of CD8+ T Cell Polyfunctionality during Chronic Hepatitis C Virus Infection
The Journal of Immunology, 2010, 184
Liu, G., Sil, D., Tong, W. H., Maio, N., Bollinger Jr, J. M., Krebs, C., & Roualult, T. A.
Dependence of heme biosynthesis on iron-sulfur cluster biogenesis: human ALAD is an unrecognized iron-sulfur protein
Nature Research, July 2020
Nelson, C. D. S., Minkkinen, E., Bergkvist, M., Hoelzer, K., Fisher, M., Bothner, B., & Parrish, C. R.
Detecting Small Changes and Additional Peptides in the Canine Parvovirus Capsid Structure
J Virol, 2008, Nov;82(21):10397-407 Epub 2008 Aug 13.
Koruzaa, B. Lafumata, Á. Végvárib, W. Knechta, S.Z. Fishera
Deuteration of human carbonic anhydrase for neutron crystallography: Cell culture media, protein thermostability, and crystallization behaviour
Archives of Biochemistry and Biophysics, March 2018
Albrethsen, J., Frederiksen, H., Andersson, A. M., Anand-Ivell, R., Nordkap, L., Bang, A. K., Jørgensen, N., Juul, A.
Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum
Clinical Chemistry and Laboratory Medicine (CCLM), doi: 10.1515/cclm-2018-0171. [Epub ahead of print], May 2018
J.L. Andersen, B. Gesser, E.D. Funder, C.J.F. Nielsen, H. Gotfred-Rasmussen, M.K. Rasmussen, et al.
Dimethyl fumarate is an allosteric covalent inhibitor of the p90 ribosomal S6 kinases
Nature Communications, volume 9, Article number: 4344, October 2018
Sampson, Jelena Zaitseva, Maria Stauffer, Brian Vande Berg, Rong Guo, Daniel Tomso, Brian McNulty, Nalini Desai, Deepa Balasubramanian
Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera
Journal of Invertebrate Pathology, October 2016
Sheila Maibom-Thomsen, Søren Heissel, Ejvind Mørtz, Peter Højrup, Jakob Bunkenborg
Discrimination of Isoleucine and Leucine by Dimethylation-Assisted MS3
Analytical Chemistry, 2018, 90 (15), 9055-9059, August 2018
Ugarte-Berzal, E. Martens, L. Boon, J. Vandooren, D. Blockmans, P. Proost, G. Opdenakker
EDTA/gelatin zymography method to identify C1s versus activated MMP‐9 in plasma and immune complexes of patients with systemic lupus erythematosus
Cell Mol. Med., September 2008
Maryam Hassan, Dag Anders Brede, Dzung B. Diep, Ingolf F. Nes, Farzaneh Lotfipour, Zoya Hojabri
Efficient Inactivation of Multi-Antibiotics Resistant Nosocomial Enterococci by Purified Hiracin Bacteriocin
Advanced Pharmaceutical Bulletin, 5(3), 393-401, September 2015
Julia Garbe, Jonathan Sjögren, Eoin F. J. Cosgrave, Weston B. Struwe, Marta Bober, Anders I. Olin, Pauline M. Rudd, Mattias Collin
EndoE from Enterococcus faecalis Hydrolyzes the Glycans of the Biofilm Inhibiting Protein Lactoferrin and Mediates Growth
PLoS ONE, March 2014
Kathrin Mutze, Sarah Vierkotten, Jadranka Milosevic, Oliver Eickelberg, Melanie Königshoff
Enolase 1 (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells
Disease Models and Mechanisms, 8: 877-890, May 2015
Mahdi Ghavami, Takehiko Shiraishi, Peter E. Nielsen
Enzyme-Triggered Release of the Antisense Octaarginine-PNA Conjugate from Phospholipase A2 Sensitive Liposomes
ACS Appl. Bio Mater., Volume 3, Issue 2, January 2020
Salganik, M., Venkatakrishnan, B., Bennett, A., Lins, B., Yarbrough, J., Muzyczka, N., Agbandje-McKenna, M., & McKenna, R.
Evidence for pH Dependent Protease Activity in the Adeno-associated Virus Capsid
Journal of Virology, 2012
Tu Anh Nguyen, Jamie Greig, Asif Khan, Cara Goh, Gregory Jedd
Evolutionary novelty in gravity sensing through horizontal gene transfer and high-order protein assembly
PLOS Biology, April 2018
S.M. Schenstrøm, C.A. Rebula, M.H. Tatham et al.
Expanded Interactome of the Intrinsically Disordered Protein Dss1
Cell Reports, Volume 25, Issue 4, October 2018
Magda E. Alvarado, Claudia Rubiano, Eliana Calvo, Vanessa Gómez & Moisés Wasserman
Experimental and bioinformatic characterization of CaBP2933 an EF-Hand protein of Giardia intestinalis
Molecular and Biochemical Parasitology, March 2017
Tanya Jelacic, Donald Chabot, Joel Bozue, Steven Tobery, Michael West, Krishna Moody, De Yang, Joost Oppenheim, Arthur Friedlander
Exposure to Bacillus anthracis capsule results in suppression of human monocyte-derived dendritic cells
American Society for Microbiology, June 2014
Julia L. Cook, Akannsha Singh, Dawn deHaro, Jawed Alam, Richard N Re
Expression of a Naturally-Occurring Angiotensin AT1 Receptor Cleavage Fragment Elicits Caspase-Activation and Apoptosis
Am J Physiol Cell Physiol, August 2011
Irina Kalbina, Lars Engstrand, Sören Andersson, Åke Strid
Expression of Helicobacter pylori TonB Protein in Transgenic Arabidopsis thaliana: Toward Production of Vaccine Antigens in Plants
Helicobacter, 2010, Vol. 15, Issue 5, pp. 430–437
Sørensen HP, Jacobsen J, Nielbo S, Poulsen FM, Wewer UM
Expression, purification and insights into structure and folding of the ADAM22 pro domain
Protein Expr Purif., October 2008, 61(2):175-83. Epub 2008 Jun 13
Shigeki Suzukia, Ashok B. Kulkarni
Extracellular heat shock protein HSP90β secreted by MG63 osteosarcoma cells inhibits activation of latent TGF-β1
Biochemical and Biophysical Research Communications
Kojima, Y. Machida, S. Palani et al.
FAM111A protects replication forks from protein obstacles via its trypsin-like domain
Nature Communications, volume 11, Article number: 1318, March 2020
Chai, S. C., Wang, W. L., & Ye, Q. Z.
FE(II) Is the Native Cofactor for Escherichia coli Methionine Aminopeptidase
Biol. Chem., Vol. 283, Issue 40, 26879-26885, October 3, 2008
Balajikarthick Subramanian, Justin Chun, Chandra Perez-Gill, Paul Yan, Isaac E. Stillman, Henry N. Higgs, Seth L. Alper, Johannes S. Schlöndorff, Martin R. Pollak
FSGS-Causing INF2 Mutation Impairs Cleaved INF2 N-Fragment Functions in Podocytes
Journal of the American Society of Nephrology, Vol. 31, Issue 2, February 2020
Martha Kampp Thomsen, Gert H. Hansen, Michael Danielsen
Galectin-2 at the enterocyte brush border of the small intestine
Molecular Membrane Biology, July 2009, Vol. 26, Issue 5, pp. 347-355
de Miranda, J. R., Dainat, B., Locke, B., Cordoni, G., Berthoud, H., Gauthier, L., Neumann, P., Budge, G. E., Ball, B. V., & Stoltz, D. B.
Genetic characterization of slow bee paralysis virus of the honeybee (Apis mellifera L.)
J Gen Virol, 2010, 91, 2524-2530
Bozhokina, E., Khaitlina, S., & Adam, T.
Grimelysin, a novel metalloprotease from Serratia grimesii, is similar to ECP32
Biochem Biophys Res Commun., 2008, Mar 21;367(4):888-92. Epub 2008 Jan 9.
Yu-Ching Su, Farshid Jalalvand, Matthias Mörgelin, et al.
Haemophilus influenzae acquires vitronectin via the ubiquitous Protein F to subvert host innate immunity
Molecular Microbiology, 2013
Kutlara, L.M. Hilliardb, L. Zhuanga, N. Patela, B. Erogluc, S.E. Meilerd, H. Carmichaela, R.B. Russelle, A. Kutlara
Hb M Dothan [ß 25/26 (B7/B8)/(GGT/GAG?GAG//Gly/Glu?Glu]; a new mechanism of unstable methemoglobin variant and molecular characteristics
Blood Cells, Molecules, and Diseases, November-December 2009, Vol. 43, Issue 3, Pp. 235-238
Liu, G., Sil, D., Maio, N., et al.
Heme biosynthesis depends on previously unrecognized acquisition of iron-sulfur cofactors in human amino-levulinic acid dehydratase
Nature Communications, volume 11, 6310, December 2020
Trana, T., Büscherc, P., Vandenbussched, G., Wynsa, L., Messensa, J., & De Grevea, H.
Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75
Journal of Biotechnology, Volume 135, Issue 3, 30 June 2008, Pages 247-254
YL Tutterrow, M Avril, K Singh, et al.
High Levels of Antibodies to Multiple Domains and Strains of VAR2CSA Correlate with Absence of Placental Malaria in Cameroonian Women Living in a High Plasmodium falciparum Transmission Area
Infection and Immunity, 2012
Lindholt, J. S., Madsen, M., Kirketerp-Møller, K. L., Schlosser, A., Kristensen, K. L., Andersen, C. B., & Sorensen, G. L.
High plasma microfibrillar-associated protein 4 is associated with reduced surgical repair in abdominal aortic aneurysms
Journal of Vascular Surgery, November 2019
Zhirong Fu, Michael Thorpe, Rahel Alemayehu, Ananya Roy, Jukka Kervinen, Lawrence de Garavilla, Magnus Åbrink, Lars Hellman
Highly Selective Cleavage of Cytokines and Chemokines by the Human Mast Cell Chymase and Neutrophil Cathepsin G
J Immunol, January 2017, 1601223
Emma Renman, Rifat Ekici, Mia Sundström & Kristina Lejon
HSC70 is a novel binding partner involved in the capture of immunoglobulins on B cells in the NOD mouse
Autoimmunity, Volume 56, August 2022
Isabella M. Y. Zanini, Charlotte Soneson, Luca E. Lorenzi & Claus M. Azzalin
Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion
Cell Sci., December 2016
Lene Udby, Anders H. Johnsen, Niels Borregaard
Human CRISP-3 binds serum a1B-glycoprotein across species
Biochimica et Biophysica Acta (BBA) – General Subjects, April 2010, Vol. 1800, Issue 4, pp. 481-485
Xue-hai Liang, Hong Sun, Wen Shen and Stanley T. Crooke
Identification and characterization of intracellular proteins that bind oligonucleotides with phosphorothioate linkages
Nucleic Acids Research, February 2015
Christoffer A Hagemann et al.
Identification and metabolic profiling of a novel human gut-derived LEAP2 fragment
The Journal of Clinical Endocrinology & Metabolism, dgaa803, November 2020
TP Schmidt, AM Perna, T Fugmann, M Böhm, J Hiss, S Haller, C Götz, N Tegtmeyer, B Hoy, TT Rau, D Neri, S Backert, G Schneider, S Wesslerb
Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA
Sci Rep., 2016; 6: 23264. March 2016
Nienke W. M. de Jong, Kasra X. Ramyar, Fermin E. Guerra, Reindert Nijland, Cindy Fevre, Jovanka M. Voyich, Alex J. McCarthy, Brandon L. Garcia, Kok P. M. van Kessel, Jos A. G. van Strijp, Brian V. Geisbrecht & Pieter-Jan A. Haas
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Vibrio cholerae Utilizes Direct sRNA Regulation in Expression of a Biofilm Matrix Protein
PLOS ONE, July 2014
Lisa Kunsmann, Christian Rüter, Andreas Bauwens, Lilo Greune, Malte Glüder, Björn Kemper, Angelika Fruth, Sun Nyunt Wai, Xiaohua He, Roland Lloubes, M. Alexander Schmidt, Ulrich Dobrindt, Alexander Mellmann, Helge Karch & Martina Bielaszewska
Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104 outbreak strain
Scientific Reports, 5, Article number: 13252, August 2015
Lan Guan & Parameswaran Hariharan
X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB
Communications Biology, volume 4, Article number: 931, August 2021
Bent Honoré, Henrik Vorum, Anders Elm Pedersen, Søren Buus, and Mogens H. Claesson
Changes in protein expression in p53 deleted spontaneous thymic lymphomas
Experimental Cell Research 295 (2004) 91– 101
Thelle K, Christensen EI, Vorum H, Ørskov H, Birn H.
Characterization of proteinuria and tubular protein uptake in a new model of oral L-lysine administration in rats.
Kidney Int. 2006 Apr;69(8):1333-40.
Nadja Larsen, Mette Boye, Henrik Siegumfeldt, and Mogens Jakobsen
Differential Expression of Proteins and Genes in the Lag Phase of Lactococcus lactis subsp. lactis Grown in Synthetic Medium and reconstituted Skim Milk.
Applied and Environmental Microbiology, 72, (2006), 1173-1179
Søren Heissel, Sigurd J. Frederiksen, Jakob Bunkenborg, Peter Højrup
Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications.
PloS one, June 2019
Henrik Vorum, Morten Østergaard, Greg E Rice, Bent Honoré, Toke Bek
Identification of differentially regulated proteins in a patient with Leber’s Congenital Amaurosis – a proteomic study
Proteome Science, 5, (2007), p.5-5
Corinna Richter, Oindrilla Mukherjee, David Ermert, Birendra Singh, Yu-Ching Su, Vaibhav Agarwal, Anna M. Blom & Kristian Riesbeck
Moonlighting of Helicobacter pylori catalase protects against complement-mediated killing by utilising the host molecule vitronectin
Nature, Scientific Reports 6, Article number: 24391, April 2016
Oskar Thofte, Emma Mattsson, Yu Ching Su, John Thegerström, Farshid Jalalvand, Anna Marie Blom and Kristian Riesbeck
Non-typeable Haemophilus influenzae P5 binds C4b-binding protein promoting serum resistance
J Immunol May 1, 2020, 204 (1 Supplement) 82.23, May 2020
Phillip Henschke, Henrik Vorum, Bent Honoré, and Gregory Edward Rice
Protein profiling the effects of in vitro hyperoxic exposure on fetal rabbit lung
Proteomics, 6, (2006), 1957-1962
Jes Lindholt, Jette Støvring, Lars Østergaard, Sigitas Urbonavicius, Eskild Henneberg, Bent Honoré, Henrik Vorum
Serum Antibodies Against Chlamydia pneumoniae Outer Membrane Protein Cross-React With the Heavy Chain of Immunoglobulin in the Wall of Abdominal Aortic Aneurysms
Circulation 109, (2004) 2097-2102
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