ELISA reagent characterization using advanced LC-MS methods

August 2023, by Ejvind Mortz

HCP-ELISA reagent characterization using LC-MS

The most frequent challenges of HCP-ELISA's - and how to solve them 

Our observation from running a vast number of projects is that frequent challenges in ELISAs are:

❌ Low HCP coverage.
❌ Not measuring abundant HCPs.
❌ Lack of dilutional linearity.
❌ Different HCP values when using new reagents.

Unfortunately, simple 2D PAGE analysis cannot help solve these challenges. You need higher-resolution analytical methods like advanced LC-MS techniques.

  • LC-MS-based ELISA reagent characterization enables a detailed understanding of the HCP-ELISA reagents and their suitability to the specific manufacturing process and purified drug substance.
  • It also enables bridging between ELISA reagents and explains differences in HCP measurements between old and new reagents.

Talk to us

Whatever protein-related challenge or question you may have, we would love to help. Our experts can help you decide on the best analytical approach for your project by email or online meeting - providing advice without obligation.

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