?

Analysis results show the accession number, the name of the protein and the formatted amino acid sequence. The colorize function can be used to highlight specific residues.

GPMAW lite logo
Enter Sequence
Get sequence from NCBI / UNIPROT
?

Type a protein accession number from NCBI or UniProt, e.g. "GI: 30794280" or "P02768". . Press Enter to retrieve the protein amino acid sequence.

Find accession number at
?

Transfer to the website site of NCBI or UNIPROT in a new window. On these websites you can easily look up accession numbers for any protein.

NCBI
UNIPROT
Paste/write sequence directly
?

Paste or type in the protein/peptide sequence using 1-letter code.


?

The CLEAN function removes all non-amino acid characters, i.e. numbers, spaces and special signs from the sequence, and turns lowercase letters into uppercase. The RESET function will clear all entries.

Choose Analysis
?

Check boxes to select the analysis you would like to have performed on your protein sequence.

?

Protein information provides basic information about the protein - Molecular Weight (mono-isotopic and average mass of non-reduced protein), theoretic calculated molar absorbance and extinction coefficient, isoelectric point and hydrophobicity index.

?

Composition shows an amino acid composition table with numbers and % of individual amino acids. If the sequence contains any unusual amino acids this will also be shown in the table.

?

Protease digest shows a list of peptides formed by theoretical complete digestion using specific proteases. The table contains info about the peptide sequence, MH+ (mono-isotopic molecular weight of the protonated peptide in reduced form), pI and expected retention time in a HPLC run (reverse phase) for each peptide. All columns can be sorted by clicking the header of the column.




Protease Digest








 

Blog posts

Useful Protein Molecular Weight Calculator

Protein molecular weight calculator The rule of thumb for conversion of microgram amounts into...

Ejvind Mørtz

GPMAW lite - Protein Molecular Weight Calculator and much more

Simple bioinformatics calculations on specific protein sequences When working with proteins...

Thomas Kofoed

USER GUIDE GPMAW LITE
BUY FULL VERSION
Analysis results
?

Analysis results show the accession number, the name of the protein and the formatted amino acid sequence. The colorize function can be used to highlight specific residues.

Protein name:

Accession number:

Colorize
Conversions
?

Select conversion from gram to mole or vice versa. The conversion is made on the fly. Based on MW of the entered sequence.




pmol 0 ng